The N-terminal sequence of HIV1 gp41(amino acid residues 584-623) was known to be the immundominant region of HEV1 gp41 protein. In order to determine epitope for gp-41 protein of Korean anti-HIV1 positive sera, multiple antigenic peptides (MAPs)
for
the sequences corresponding to 584-612, 590-612, 604-623 and 584-618 of HIV1 gp41 were synthesized by solid phase method using Fmoc-Lys (Fmoc)-OH and used as coating antigens for ELISA. The reactivities of the syntetic peptides with Korean HIV1
positive
(21 samples) and anti-HIV1 negative sera (22 samples) obtained from healthy blood doner were estimated by an indirect ELISA. MAPs for 584-604, 590-612 and 604-623 of gp-41 reacted with 62%, 100% and 81% of Korean anti-HIV1 positive sera tested,
respectively. The results suggest that the epitope for HIV-1 gp 41 for Korean anti-HIV1 positive sera is located in the region of amino acid 590-612 of gp41 for Korean anti-GIV1 positive sera is located in the region of amio acid 590-612 of gp41.
MAP
for gp41 (548-618) reacted with all(100%) of anti-HIV1 positive tested, but did not react with antiHIV1 negative sera. In addition, this MAP reacted stronger with seven samples of anti-HIV1 positive sera of anti-HIV1/2 combo performance panel
than
the
mixture of 584-604, 590-612 and 604-623 of gp41, but did not react with anti-HIV negative serum. The high in an ELISA system will be useful for antibody detection of HIV1.
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